Flavonoid profiling in three citrus varieties native to the Republic of Korea using liquid chromatography coupled with tandem mass spectrometry: contribution to overall antioxidant activity
Identifieur interne : 001495 ( Main/Exploration ); précédent : 001494; suivant : 001496Flavonoid profiling in three citrus varieties native to the Republic of Korea using liquid chromatography coupled with tandem mass spectrometry: contribution to overall antioxidant activity
Auteurs : Hae Gyeong Kim [Corée du Sud] ; Gon-Sup Kim [Corée du Sud] ; Semin Park [Corée du Sud] ; Jung Han Lee [Corée du Sud] ; On Nuri Seo [Corée du Sud] ; Soo Jung Lee [Corée du Sud] ; Jae Hoon Kim [Corée du Sud] ; Jae-Han Shim [Corée du Sud] ; A. M. Abd El-Aty [Égypte] ; Jong Sung Jin [Corée du Sud] ; Sung Chul Shin [Corée du Sud]Source :
- Biomedical Chromatography [ 0269-3879 ] ; 2012-04.
Abstract
A mixture of flavonoid components was isolated from the fruit peel of three varieties of citrus native to Republic of Korea, Citrus leiocarpa Hort. ex Tanaka (CLHT), Citrus aurantium L. (CAL) and Citrus erythrosa Hort. (CEH), via 70% methanol extraction followed by ethyl acetate elution over a silica gel cartridge. The flavonoid components of the mixture were analyzed via high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) in positive‐ion mode and a comparison of the reported data. Among 17 characterized components, two flavanones, four flavones and two coumarin derivatives in the fruit peel of the three varieties were identified for the first time. The individual characterized components were quantified via HPLC‐UV. The flavanones dominated in CAL, whereas the flavones prevailed in CLHT and CEH. The antioxidant activity of the flavonoid mixture of the fruit peel was determined via DPPH•, ABTS•+ and reducing power assays. The antioxidant activity of CEH and CAL was greater than that of CLHT. Copyright © 2011 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/bmc.1688
Affiliations:
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Abd El-Aty</name><affiliation wicri:level="1"><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza</wicri:regionArea><wicri:noRegion>Giza</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Jong Sung" sort="Jin, Jong Sung" uniqKey="Jin J" first="Jong Sung" last="Jin">Jong Sung Jin</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of High Technology Materials Research, Busan Center, Korea Basic Science Institute (KBSI), 618‐230, Busan</wicri:regionArea><wicri:noRegion>Busan</wicri:noRegion></affiliation></author><author><name sortKey="Shin, Sung Chul" sort="Shin, Sung Chul" uniqKey="Shin S" first="Sung Chul" last="Shin">Sung Chul Shin</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, 660‐701, Jinju</wicri:regionArea><wicri:noRegion>Jinju</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Corée du Sud</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Biomedical Chromatography</title><title level="j" type="abbrev">Biomed. Chromatogr.</title><idno type="ISSN">0269-3879</idno><idno type="eISSN">1099-0801</idno><imprint><publisher>John Wiley & Sons, Ltd</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2012-04">2012-04</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="464">464</biblScope><biblScope unit="page" to="470">470</biblScope></imprint><idno type="ISSN">0269-3879</idno></series><idno type="istex">DF3B9F8EAF9AD211508AE7E49505B12203C2DF4D</idno><idno type="DOI">10.1002/bmc.1688</idno><idno type="ArticleID">BMC1688</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0269-3879</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">A mixture of flavonoid components was isolated from the fruit peel of three varieties of citrus native to Republic of Korea, Citrus leiocarpa Hort. ex Tanaka (CLHT), Citrus aurantium L. (CAL) and Citrus erythrosa Hort. (CEH), via 70% methanol extraction followed by ethyl acetate elution over a silica gel cartridge. The flavonoid components of the mixture were analyzed via high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) in positive‐ion mode and a comparison of the reported data. Among 17 characterized components, two flavanones, four flavones and two coumarin derivatives in the fruit peel of the three varieties were identified for the first time. The individual characterized components were quantified via HPLC‐UV. The flavanones dominated in CAL, whereas the flavones prevailed in CLHT and CEH. The antioxidant activity of the flavonoid mixture of the fruit peel was determined via DPPH•, ABTS•+ and reducing power assays. The antioxidant activity of CEH and CAL was greater than that of CLHT. Copyright © 2011 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li><li>Égypte</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Kim, Hae Gyeong" sort="Kim, Hae Gyeong" uniqKey="Kim H" first="Hae Gyeong" last="Kim">Hae Gyeong Kim</name></noRegion><name sortKey="Jin, Jong Sung" sort="Jin, Jong Sung" uniqKey="Jin J" first="Jong Sung" last="Jin">Jong Sung Jin</name><name sortKey="Kim, Gon Up" sort="Kim, Gon Up" uniqKey="Kim G" first="Gon-Sup" last="Kim">Gon-Sup Kim</name><name sortKey="Kim, Jae Hoon" sort="Kim, Jae Hoon" uniqKey="Kim J" first="Jae Hoon" last="Kim">Jae Hoon Kim</name><name sortKey="Lee, Jung Han" sort="Lee, Jung Han" uniqKey="Lee J" first="Jung Han" last="Lee">Jung Han Lee</name><name sortKey="Lee, Soo Jung" sort="Lee, Soo Jung" uniqKey="Lee S" first="Soo Jung" last="Lee">Soo Jung Lee</name><name sortKey="Park, Semin" sort="Park, Semin" uniqKey="Park S" first="Semin" last="Park">Semin Park</name><name sortKey="Seo, On Nuri" sort="Seo, On Nuri" uniqKey="Seo O" first="On Nuri" last="Seo">On Nuri Seo</name><name sortKey="Shim, Jae An" sort="Shim, Jae An" uniqKey="Shim J" first="Jae-Han" last="Shim">Jae-Han Shim</name><name sortKey="Shim, Jae An" sort="Shim, Jae An" uniqKey="Shim J" first="Jae-Han" last="Shim">Jae-Han Shim</name><name sortKey="Shin, Sung Chul" sort="Shin, Sung Chul" uniqKey="Shin S" first="Sung Chul" last="Shin">Sung Chul Shin</name><name sortKey="Shin, Sung Chul" sort="Shin, Sung Chul" uniqKey="Shin S" first="Sung Chul" last="Shin">Sung Chul Shin</name></country><country name="Égypte"><noRegion><name sortKey="Abd El Ty, A M" sort="Abd El Ty, A M" uniqKey="Abd El Ty A" first="A. M." last="Abd El-Aty">A. M. Abd El-Aty</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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